Eggs lose from 13 to 16 per cent of their moisture during incubation are said to undergo normal evaporation. Infertile eggs oddly enough, evaporate faster than do those containing live germs; hence the candling or testing of the eggs is important, because keeping the humidity level is important.
I start checking the eggs on day #5. This day is the first day after the embryo has formed and is available for viewing. If the eggs are infertile at this point, typically this is a cockbirds problem. It’s important to know as soon as possible otherwise you can, and I have before I knew this, waste a lot of time to get a bad hatch.
The next time I check is on day #19 when i move them from the incubator to the hatcher.